ABSTRACT
Une new flavonoids named as notabilisin K (1), together with four known compounds, morusin (2), mulberrofuran A (3), neocyclomorusin (4) and mornigrol F (5) are separated from 95% ethanol extracts of the twigs of Morus notabilis. Compounds 2-5 are separated from this plant for the first time. Notabilisin I, notabilisin J exhibits certain effect against cells of HCT-116, HepG2 and A2780 with IC50 values ranging from 1.47 μmol x L(-1) to 5.46 μmol x L(-1). Morusin exhibits strong effect against five kinds of human cancer cells (BGC823, A2780, HCT-116, HepG2 and NCI-H1650) with IC50 values ranging from 0.74 μmol x L(-1) to 1.58 μmol x L(-1).
ABSTRACT
Une new flavonoids named as notabilisin K (1), together with four known compounds, morusin (2), mulberrofuran A (3), neocyclomorusin (4) and mornigrol F (5) are separated from 95% ethanol extracts of the twigs of Morus notabilis. Compounds 2-5 are separated from this plant for the first time. Notabilisin I, notabilisin J exhibits certain effect against cells of HCT-116, HepG2 and A2780 with IC50 values ranging from 1.47 μmol x L(-1) to 5.46 μmol x L(-1). Morusin exhibits strong effect against five kinds of human cancer cells (BGC823, A2780, HCT-116, HepG2 and NCI-H1650) with IC50 values ranging from 0.74 μmol x L(-1) to 1.58 μmol x L(-1).
Subject(s)
Humans , Antineoplastic Agents, Phytogenic , Chemistry , Benzofurans , Chemistry , Flavonoids , Chemistry , Hep G2 Cells , Inhibitory Concentration 50 , Morus , Chemistry , Plant Extracts , Chemistry , Terpenes , ChemistryABSTRACT
Several factors influencing the extraction of tetrandrine and demethyltetrandrine from Chinese medicinal herbs, Stephania tetrandra S. Moore were studied by means of Ortogonal method. The optimum Condition was eight hours steeping, in 33.3% ammonia in absolute alcohol for four hours under ultrasonic agitation. Tctrandrine aud demethyltetrandrino were detected by HPLC. The contents were 0.678% and 0.616%, the detection limits were 3.87?10-3and 0.48?10-3 mmol/L respectively.
ABSTRACT
AIM: To establish HPLC fingerprint of Sishen Pill and a determination of main component of Sishen Pills(Fructus Evodiae, Semen Myristicae, Fructus Psoraleae, etc.) by HPLC. METHODS: The samples were extracted with the solvent methanol. The separation was performed on YWG-C 18(4.6mm?250mm, 10?m) column with water- methanol-acetonitrile as a mobile phase gradient elution. The flow rate was 1.0mL?min -1, the column temperature was at 30℃ and the wavelength of UV detector was at 225nm. RESULTS: There are 21 higher peaks and 12 marker peaks in the fingerprint of eight batches of samples. For evodiamine, the regression equation was Y=147.37X+1186.32, r=0.9999, the liner range was from 5 to 1000?g?mL -1 and the recovery was 99.83%. For rutaecarpin, the regression equation was Y=101.19X+279.81, r= 0.9999, the liner range was from 4.5 to 900?g?mL -1. The recovery was 98.72%. CONCLUSION: The quality standard of Sishen Pill was established firstly. It can be used for quality cantrol of Sishen Pill.
ABSTRACT
Objective: Assay of amygdalin of Ephedra spp. and Apricot Kernel Cough Lozenge (ephedrine hydrochloride, Semen Armeniacae Amarum, etc.) by HPLC. Methods: Amygdalin was extracted with methanol. The separation was performed by HPLC and amygdalin was determined with UV detector. Results: The contents of amygdalin of Ephedra spp. and Apricot Kernel Cough Lozenge was 0.476% and 1.53mg per lozenge. Conclusion: The method was simple, accurate, rapid and with good reproducibility. It can be used for quality control of Ephedra spp. and Apricot Kernel Cough Lozenge.